by Yuix

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• Within each population average copy number across individuals was calculated for every given interval and transformed to log2 values.
• Moreover, some samples were provided by collaborators who have only recorded the general area for trapping, but not the exact location (e.g., the mice from Kazakhstan).
• The ride is one of three in American Disney Parks taking the names of former Opryland USA attractions.
• Here, we introduced the role of EVs in OA progression by influencing inflammation, metabolism, and aging.
• One of those companies was run by a savvy young group of entrepreneurs including Bumper McFerrin (a rafting instructor who provided the attraction’s safety spiel).
• Finally, we discuss the deficiencies and put forward the prospects and challenges related to the application of EVs in the field of OA.

We used the NEBNext Ultra RNA Library Prep Kit for Illumina with the Poly(A) mRNA Magnetic Isolation module to generate the RNAseq libraries. We used a fragmentation time of 15 min (yielding ~180 bp fragments) and 15 PCR cycles for library enrichment. After validation (Agilent 2,200 TapeStation), all libraries were quantified using the Peqlab KAPA Library Quantification Kit and the Applied Biosystems 7900HT Sequence Detection System.

Lighting

The house mouse (Mus musculus) has a long-standing history as a model system in genetics and biomedical research, with many classical inbred strains available for purchase worldwide. Classical inbred strains were found to exhibit a much reduced amount of genetic variation compared to their wild mice ancestors10. For example, all classical inbred strains share a single mitochondrial lineage derived from M. Domesticus11, indicating that they all descend from the same female lineage of the wild ancestor. The Indian population is especially informative, as it has been extensively sequenced51,72.

We use a single pipeline to map and call variants for these individuals and also include 10 additional individuals of M. Castaneus for which genomic data are publically available. In addition, RNAseq data were obtained from 10 tissues of up to eight adult individuals from each of the three M. Domesticus populations for which genomic data were collected. Data and analyses are presented via tracks viewable in the UCSC or IGV genome browsers.

Supplementary Table 3 (XLSX 66 kb)

All mice were fed standard mouse chow ad libitum (Altromin 1,324 from ALTROMIN, 32,791 Lage, Germany). Mice were sacrificed by CO2 https://slot-online.com/pg-slot/candy-burst/ asphyxiation. The coat was sprayed with 75% EtOH to reduce loose hair contaminating the organs during dissection of the animal.

Aquarius mermaid

Other trapping locations had regional limitations. For example, the island of Heligoland is only 1.7 km2 in size, or the military Camp Marmal, Mazar-e-Sharif (Afghanistan) is only 8.7 km2 in size. In both cases, mice were collected in different localities on the island or military base respectively50. We used the sequencing read depth approach implemented in the CNVnator software63 to predict CNV calls relative to the mouse mm10 reference assembly. We have previously experimentally confirmed that this is a reliable approach38.

Here we describe the genomic resources that we have generated using mice collected in the wild over the past 10 years. These data can serve as a basis for in depth studies at a population level and to inform biomedical research projects on natural polymorphisms that are present in inbred strains. We provide information on a) genomic data for a total of nine populations (Fig. 1), covering the three major house mouse subspecies and one outgroup, b) tissue-specific RNAseq data from three M.

For two additional Iranian individuals in the DNA sequencing study, tissue samples were lost and thus no corresponding RNAseq data exist. To compensate for this, we included four individuals representing male offspring from male AH15 and male AH23 respectively (both individuals are part of the DNAseq dataset; see Table 1 (available online only)). Two male offspring from each of these two males are represented as biological replicates in the RNAseq dataset.